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Image Search Results
Journal: eLife
Article Title: Dopamine drives Drosophila sechellia adaptation to its toxic host
doi: 10.7554/eLife.03785
Figure Lengend Snippet: ( A – C ) Confocal images showing striking accumulation (arrowhead) of TH-PLE (green, anti-TH) in D. sechellia (14021–0248.25) ( B ) and D. melanogaster DGRP-357 ( C ), compared to in D. melanogaster wild-type Berlin (wtB) ( A ) oocytes. ( D – F ) Confocal images showing CATSUP (magenta, anti-SLC39A7) expressed in the nurse cells (arrow) of D. melanogaster wild-type Berlin (wtB) oocytes ( D ) and absent in the nurse cells of D. sechellia (14021–0248.25, sec) ( E ) and D. melanogaster DGRP-357 (DGRP-357) ( F ) oocytes. ( G – I ) Confocal image showing CATSUP expressed in the nuclei (arrow) and the membrane (arrowhead) of D. melanogaster wild-type Berlin (wtB) spermatheca secretory cells ( G ), and absent from D. sechellia (14021–0248.25) ( H ) and D. melanogaster DGRP-357 (DGRP-357) ( I ). Scale bar 20 μm. DOI: http://dx.doi.org/10.7554/eLife.03785.018
Article Snippet: After being blocked in 5% non-fat milk in TBS-tween (TBS, 0.05% Tween-20 [Sigma, St. Louis, MO]) for 2 hr, at RT, membranes were incubated with 1:1000 dilutions of primary antibodies (anti-TH [mouse, ImmunoStar, Acris Antibodies, GmbH, Germany], or
Techniques: